Fig 1: SLC39A7 played a vital role in cell survival of lung adenocarcinoma. (A) CERES values of SLC39A7 in various lung adenocarcinoma cell lines, the score less than -1 means the gene is important for cell survival. (B) After 48 h transfected with control siRNA or siRNA-SLC39A7, A549 cells were harvested. The lysates were undergone for detecting SLC39A7 by Western blotting. (C) After seeding, A549 cells were transfected with control siRNA or siRNA-SLC39A7. The CCK-8 assay was performed at 0.5, 1.5, 2.5 and 3.5 days after transfection. The cell viability of siRNA-SLC39A7 treatment was normalized to control group. (D) A549 cells were transfected with control siRNA or siRNA-SLC39A7. Then the quantitative cells were placed and cultured for 14 day, and stained with crystal violet-glutaraldehyde solution. All experiments performed in triplicate, and the results are calculated using mean ± standard deviation. One-way ANOVA or Student t test was carried out for evaluating the statistical significance between groups. *** P<0.001. SLC39A, solute carrier family 39.
Fig 2: SLC39A7 expression in lung adenocarcinoma was associated with smoking statues. TCGA-LUAD dataset was downloaded and analyzed. (A) Expression of SLC39A7 in current smokers or lifelong non-smokers. (B) Expression of SLC39A7 in current smokers or history smokers. (C) Expression of SLC39A7 between lifelong non-smokers, history smokers (>15 y) and history smokers (=15 y). (D) SLC39A7 expression based on smoking duration time (y) analyzed by using UCSC databases. SLC39A, solute carrier family 39.
Fig 3: Protein expression of SLC39A genes in patients with lung adenocarcinoma. (A) SLC39A4. (B) SLC39A6. (C) SLC39A7. (D) SLC39A8. (E) SLC39A10. (F) SLC39A11. (G) SLC39A14. (H) Immunohistochemical staining of SLC39A7 proteins in lung adenocarcinoma tissue specimens. * P<0.05; SLC39A, solute carrier family 39.
Fig 4: GO enriched analysis, GSEA analysis and co-expression analysis of SLC39A7 in lung adenocarcinoma. (A) Heat-map showed the DEGs of SLC39A7. (B) GO enriched analysis (CC) for SLC39A7. (C) GSEA of Wnt/ß-catenin signaling pathway. (D) Co-expression of SLC39A7 and RRBP1 by using cBioportal database. (E) RRBP1 expression of mRNA in LUAD tissues and relative normal tissues in Ualcan database. (F) Kaplan-Meier plotter was used to evaluate prognostic value (OS) of RRBP1 in lung adenocarcinoma. A549 cells were transfected with a control siRNA or siRNA-SLC39A7 (G)/ RRBP1 (H). Western blotting was performed to detect the expression of SLC39A7 and RRBP1. (I) Immunohistochemistry assays were performed to detect expression and distribution of SLC39A7 and RRBP1 proteins in lung adenocarcinoma tissues. DEG, differentially expressed genes; GSEA, Gene Set Enrichment Analysis; RRBP1, ribosome binding protein 1; SLC39A, solute carrier family 39.
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